mouse anti major histocompatibility complex ii Search Results


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T-MSCs inhibit differentiation and maturation of DCs under coculture conditions. Bone-marrow- (BM-) derived monocytes (BMCs) isolated from 8-week-old BALB/c mice were incubated with of granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng/mL) for 12 days to induce differentiation into dendritic cells (DCs). (a) Lipopolysaccharide (LPS) (1 μ g/mL) was added for the last 2 days to induce DC maturation. (b) GM-CSF treatment induced expansion of CD11b + cells ( ** P < 0.01). (c) Tonsil-derived MSCs (T-MSCs) added at day 0, but not at day 10, inhibited upregulated expression of CD80 ( * P < 0.05) and CD86 ( ** P < 0.01) on mature DCs by direct contact. Upregulated major histocompatibility complex <t>(MHC)</t> class II expression on immature ( ** P < 0.01) and mature DCs ( ** P < 0.01) was reduced by T-MSCs, <t>but</t> <t>CD14</t> expression was not affected.
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T-MSCs inhibit differentiation and maturation of DCs under coculture conditions. Bone-marrow- (BM-) derived monocytes (BMCs) isolated from 8-week-old BALB/c mice were incubated with of granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng/mL) for 12 days to induce differentiation into dendritic cells (DCs). (a) Lipopolysaccharide (LPS) (1 μ g/mL) was added for the last 2 days to induce DC maturation. (b) GM-CSF treatment induced expansion of CD11b + cells ( ** P < 0.01). (c) Tonsil-derived MSCs (T-MSCs) added at day 0, but not at day 10, inhibited upregulated expression of CD80 ( * P < 0.05) and CD86 ( ** P < 0.01) on mature DCs by direct contact. Upregulated major histocompatibility complex <t>(MHC)</t> class II expression on immature ( ** P < 0.01) and mature DCs ( ** P < 0.01) was reduced by T-MSCs, <t>but</t> <t>CD14</t> expression was not affected.
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Becton Dickinson fitc-conjugated mouse anti-human major histocompatibility (mhc) class (hla-abc
T-MSCs inhibit differentiation and maturation of DCs under coculture conditions. Bone-marrow- (BM-) derived monocytes (BMCs) isolated from 8-week-old BALB/c mice were incubated with of granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng/mL) for 12 days to induce differentiation into dendritic cells (DCs). (a) Lipopolysaccharide (LPS) (1 μ g/mL) was added for the last 2 days to induce DC maturation. (b) GM-CSF treatment induced expansion of CD11b + cells ( ** P < 0.01). (c) Tonsil-derived MSCs (T-MSCs) added at day 0, but not at day 10, inhibited upregulated expression of CD80 ( * P < 0.05) and CD86 ( ** P < 0.01) on mature DCs by direct contact. Upregulated major histocompatibility complex <t>(MHC)</t> class II expression on immature ( ** P < 0.01) and mature DCs ( ** P < 0.01) was reduced by T-MSCs, <t>but</t> <t>CD14</t> expression was not affected.
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T-MSCs inhibit differentiation and maturation of DCs under coculture conditions. Bone-marrow- (BM-) derived monocytes (BMCs) isolated from 8-week-old BALB/c mice were incubated with of granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng/mL) for 12 days to induce differentiation into dendritic cells (DCs). (a) Lipopolysaccharide (LPS) (1 μ g/mL) was added for the last 2 days to induce DC maturation. (b) GM-CSF treatment induced expansion of CD11b + cells ( ** P < 0.01). (c) Tonsil-derived MSCs (T-MSCs) added at day 0, but not at day 10, inhibited upregulated expression of CD80 ( * P < 0.05) and CD86 ( ** P < 0.01) on mature DCs by direct contact. Upregulated major histocompatibility complex <t>(MHC)</t> class II expression on immature ( ** P < 0.01) and mature DCs ( ** P < 0.01) was reduced by T-MSCs, <t>but</t> <t>CD14</t> expression was not affected.
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Becton Dickinson fitc-anti-mouse histocompatibility complex class antigen h-2k[d
T-MSCs inhibit differentiation and maturation of DCs under coculture conditions. Bone-marrow- (BM-) derived monocytes (BMCs) isolated from 8-week-old BALB/c mice were incubated with of granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng/mL) for 12 days to induce differentiation into dendritic cells (DCs). (a) Lipopolysaccharide (LPS) (1 μ g/mL) was added for the last 2 days to induce DC maturation. (b) GM-CSF treatment induced expansion of CD11b + cells ( ** P < 0.01). (c) Tonsil-derived MSCs (T-MSCs) added at day 0, but not at day 10, inhibited upregulated expression of CD80 ( * P < 0.05) and CD86 ( ** P < 0.01) on mature DCs by direct contact. Upregulated major histocompatibility complex <t>(MHC)</t> class II expression on immature ( ** P < 0.01) and mature DCs ( ** P < 0.01) was reduced by T-MSCs, <t>but</t> <t>CD14</t> expression was not affected.
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Becton Dickinson fitc-conjugated monoclonal rat anti-mouse major histocompatibility complex ii antibody
T-MSCs inhibit differentiation and maturation of DCs under coculture conditions. Bone-marrow- (BM-) derived monocytes (BMCs) isolated from 8-week-old BALB/c mice were incubated with of granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng/mL) for 12 days to induce differentiation into dendritic cells (DCs). (a) Lipopolysaccharide (LPS) (1 μ g/mL) was added for the last 2 days to induce DC maturation. (b) GM-CSF treatment induced expansion of CD11b + cells ( ** P < 0.01). (c) Tonsil-derived MSCs (T-MSCs) added at day 0, but not at day 10, inhibited upregulated expression of CD80 ( * P < 0.05) and CD86 ( ** P < 0.01) on mature DCs by direct contact. Upregulated major histocompatibility complex <t>(MHC)</t> class II expression on immature ( ** P < 0.01) and mature DCs ( ** P < 0.01) was reduced by T-MSCs, <t>but</t> <t>CD14</t> expression was not affected.
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Biozol Diagnostica Vertrieb GmbH er-tr2 (rat anti-mouse major histocompatibility complex [mhc] class ii
T-MSCs inhibit differentiation and maturation of DCs under coculture conditions. Bone-marrow- (BM-) derived monocytes (BMCs) isolated from 8-week-old BALB/c mice were incubated with of granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng/mL) for 12 days to induce differentiation into dendritic cells (DCs). (a) Lipopolysaccharide (LPS) (1 μ g/mL) was added for the last 2 days to induce DC maturation. (b) GM-CSF treatment induced expansion of CD11b + cells ( ** P < 0.01). (c) Tonsil-derived MSCs (T-MSCs) added at day 0, but not at day 10, inhibited upregulated expression of CD80 ( * P < 0.05) and CD86 ( ** P < 0.01) on mature DCs by direct contact. Upregulated major histocompatibility complex <t>(MHC)</t> class II expression on immature ( ** P < 0.01) and mature DCs ( ** P < 0.01) was reduced by T-MSCs, <t>but</t> <t>CD14</t> expression was not affected.
Er Tr2 (Rat Anti Mouse Major Histocompatibility Complex [Mhc] Class Ii, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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T-MSCs inhibit differentiation and maturation of DCs under coculture conditions. Bone-marrow- (BM-) derived monocytes (BMCs) isolated from 8-week-old BALB/c mice were incubated with of granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng/mL) for 12 days to induce differentiation into dendritic cells (DCs). (a) Lipopolysaccharide (LPS) (1 μ g/mL) was added for the last 2 days to induce DC maturation. (b) GM-CSF treatment induced expansion of CD11b + cells ( ** P < 0.01). (c) Tonsil-derived MSCs (T-MSCs) added at day 0, but not at day 10, inhibited upregulated expression of CD80 ( * P < 0.05) and CD86 ( ** P < 0.01) on mature DCs by direct contact. Upregulated major histocompatibility complex (MHC) class II expression on immature ( ** P < 0.01) and mature DCs ( ** P < 0.01) was reduced by T-MSCs, but CD14 expression was not affected.

Journal: Stem Cells International

Article Title: Immune Suppressive Effects of Tonsil-Derived Mesenchymal Stem Cells on Mouse Bone-Marrow-Derived Dendritic Cells

doi: 10.1155/2015/106540

Figure Lengend Snippet: T-MSCs inhibit differentiation and maturation of DCs under coculture conditions. Bone-marrow- (BM-) derived monocytes (BMCs) isolated from 8-week-old BALB/c mice were incubated with of granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng/mL) for 12 days to induce differentiation into dendritic cells (DCs). (a) Lipopolysaccharide (LPS) (1 μ g/mL) was added for the last 2 days to induce DC maturation. (b) GM-CSF treatment induced expansion of CD11b + cells ( ** P < 0.01). (c) Tonsil-derived MSCs (T-MSCs) added at day 0, but not at day 10, inhibited upregulated expression of CD80 ( * P < 0.05) and CD86 ( ** P < 0.01) on mature DCs by direct contact. Upregulated major histocompatibility complex (MHC) class II expression on immature ( ** P < 0.01) and mature DCs ( ** P < 0.01) was reduced by T-MSCs, but CD14 expression was not affected.

Article Snippet: For phenotyping, cells were incubated with the following fluorochrome-conjugated antibodies at final concentration of 2 ug/mL: FITC-anti-mouse CD11b (M1/70, Rat IgG 2b , eBiosciences, San Diego, CA), PE-anti-mouse CD11c (HL3, Hamster IgG 1 , BD Biosciences, Franklin Lakes, NJ), APC-anti-mouse CD11c (N418, Hamster IgG, BioLegend, San Diego, CA), PerCP-anti-mouse CD80 (16-1 OA1, Hamster IgG 2 , BD Biosciences), PE-anti-mouse CD86 (GL1, Rat IgG 2a , BD Biosciences), PE-anti-mouse CD14 (rmC5-3, Rat IgG 1 , BD Biosciences), PE-anti-mouse class II major histocompatibility complex (MHC) (2G9, Rat IgG 2a , BD Biosciences), and PE-anti-mouse CCR7 (4B12, Rat IgG 2a , R&D Systems).

Techniques: Derivative Assay, Isolation, Incubation, Expressing

T-MSCs inhibit differentiation and maturation of DCs by secretion of soluble factors. (a) BMCs and T-MSCs were cultured in a transwell plate and BMCs were induced to differentiate into DCs by GM-CSF. (b) BMCs differentiation into CD11b + cells was inhibited by T-MSCs added at day 0 ( ** P < 0.01). (c) CD86 expression on mature DCs was significantly downregulated by T-MSCs ( ** P < 0.01). Similar to the coculture condition, MHC class II expression on immature and mature DCs was inhibited by factors secreted from T-MSCs ( ** P < 0.01). CD14 expression by T-MSCs showed a trend toward increase, but it was not significant.

Journal: Stem Cells International

Article Title: Immune Suppressive Effects of Tonsil-Derived Mesenchymal Stem Cells on Mouse Bone-Marrow-Derived Dendritic Cells

doi: 10.1155/2015/106540

Figure Lengend Snippet: T-MSCs inhibit differentiation and maturation of DCs by secretion of soluble factors. (a) BMCs and T-MSCs were cultured in a transwell plate and BMCs were induced to differentiate into DCs by GM-CSF. (b) BMCs differentiation into CD11b + cells was inhibited by T-MSCs added at day 0 ( ** P < 0.01). (c) CD86 expression on mature DCs was significantly downregulated by T-MSCs ( ** P < 0.01). Similar to the coculture condition, MHC class II expression on immature and mature DCs was inhibited by factors secreted from T-MSCs ( ** P < 0.01). CD14 expression by T-MSCs showed a trend toward increase, but it was not significant.

Article Snippet: For phenotyping, cells were incubated with the following fluorochrome-conjugated antibodies at final concentration of 2 ug/mL: FITC-anti-mouse CD11b (M1/70, Rat IgG 2b , eBiosciences, San Diego, CA), PE-anti-mouse CD11c (HL3, Hamster IgG 1 , BD Biosciences, Franklin Lakes, NJ), APC-anti-mouse CD11c (N418, Hamster IgG, BioLegend, San Diego, CA), PerCP-anti-mouse CD80 (16-1 OA1, Hamster IgG 2 , BD Biosciences), PE-anti-mouse CD86 (GL1, Rat IgG 2a , BD Biosciences), PE-anti-mouse CD14 (rmC5-3, Rat IgG 1 , BD Biosciences), PE-anti-mouse class II major histocompatibility complex (MHC) (2G9, Rat IgG 2a , BD Biosciences), and PE-anti-mouse CCR7 (4B12, Rat IgG 2a , R&D Systems).

Techniques: Cell Culture, Expressing